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Presenilin-1-Specific Recombinant antibody

Presenilin-1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240615B4

Cat no : 83618-3-RR

Print datasheet

Synonyms

PSEN1, Presenilin-1 CTF12, Presenilin-1 CTF subunit, Presenilin-1, EC:3.4.23.-

验证数据展示

  • WB analysis using 83618-3-RR

    WB analysis using 83618-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83618-3-RR (PSEN1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83618-3-RR

    IHC staining of mouse brain using 83618-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSEN1/AD3 antibody included
  • IHC staining of mouse brain using 83618-3-RR

    IHC staining of mouse brain using 83618-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSEN1/AD3 antibody included
  • IF Staining of rat brain using 83618-3-RR

    IF Staining of rat brain using 83618-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 83618-3-RR

    IF Staining of rat brain using 83618-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, Caco-2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy PSEN1/AD3 Ready-To-Use IHC Kit. PSEN1/AD3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83618-3-RR targets Presenilin-1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name presenilin 1
Synonyms PSEN1, Presenilin-1 CTF12, Presenilin-1 CTF subunit, Presenilin-1, EC:3.4.23.-
Calculated Molecular Weight 53 kDa
Observed Molecular Weight53 kda
GenBank Accession NumberNM_000021
Gene Symbol PSEN1
Gene ID (NCBI) 5663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.

实验方案

Product Specific Protocols
WB protocol for Presenilin-1-Specific antibody 83618-3-RRDownload protocol
IHC protocol for Presenilin-1-Specific antibody 83618-3-RRDownload protocol
IF protocol for Presenilin-1-Specific antibody 83618-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols