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  • Featured Product
  • KD/KO Validated

ProSAPiP1 Polyclonal antibody

ProSAPiP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24936-1-AP

Print datasheet

Synonyms

ProSAPiP 1, ProSAP-interacting protein 1, LZTS3, Leucine zipper putative tumor suppressor 3, KIAA0552

验证数据展示

  • WB analysis using 24936-1-AP

    WB analysis using 24936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 24936-1-AP

    IHC staining of rat brain using 24936-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24936-1-AP

    IHC staining of mouse brain using 24936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24936-1-AP

    IF Staining of HeLa using 24936-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ProSAPiP1 antibody (24936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24936-1-AP targets ProSAPiP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ProSAPiP1 fusion protein Ag20765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ProSAPiP1 protein
Synonyms ProSAPiP 1, ProSAP-interacting protein 1, LZTS3, Leucine zipper putative tumor suppressor 3, KIAA0552
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC038860
Gene Symbol ProSAPiP1
Gene ID (NCBI) 9762
RRIDAB_2714022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
IHC protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
IF protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Neuron

Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.

Authors - Sophie Laguesse
  • KD Validated
View Article
WB

J Neurosci

Nacc1 Mutation in Mice Models Rare Neurodevelopmental Disorder with Underlying Synaptic Dysfunction

Authors - Mark A Deehan
View Article