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验证数据展示

  • WB analysis using 27094-1-AP

    WB analysis using 27094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27094-1-AP

    WB analysis of HeLa using 27094-1-AP

    WB result of RAB10 antibody (27094-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.

  • IF Staining of NIH/3T3 using 27094-1-AP

    IF Staining of NIH/3T3 using 27094-1-AP

    Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 27094-1-AP (RAB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 27094-1-AP

    IHC staining of human colon using 27094-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB10 antibody included
  • IHC staining of human colon using 27094-1-AP

    IHC staining of human colon using 27094-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB10 antibody included
  • FC experiment of HeLa using 27094-1-AP

    FC experiment of HeLa using 27094-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RAB10 (27094-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

RAB10 Polyclonal antibody

RAB10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 27094-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

别名

Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB10 Ready-To-Use IHC Kit. RAB10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27094-1-AP targets RAB10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse
免疫原 RAB10 fusion protein Ag25830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB10, member RAS oncogene family
别名 Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2
计算分子量 200 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC000896
基因名称 RAB10
Gene ID (NCBI) 10890
RRIDAB_2880752
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61026
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.

实验方案

Product Specific Protocols
WB protocol for RAB10 antibody 27094-1-APDownload protocol
IHC protocol for RAB10 antibody 27094-1-APDownload protocol
IF protocol for RAB10 antibody 27094-1-APDownload protocol
FC protocol for RAB10 antibody 27094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
View Article
mouseIF

Biochem Biophys Res Commun

Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation

Authors - Miku Fukawa
View Article