• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28498-1-AP

    WB analysis using 28498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28498-1-AP (RAB11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 28498-1-AP

    IF Staining of A549 using 28498-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11B antibody (28498-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 28498-1-AP

    IHC staining of human stomach using 28498-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28498-1-AP (RAB11B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB11B antibody included

RAB11B Polyclonal antibody

RAB11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28498-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Ras-related protein Rab-11B, Ras related protein Rab 11B, H YPT3, GTP-binding protein YPT3, GTP binding protein YPT3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, PC-12 cells, SH-SY5Y cells, RAW 264.7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, NIH3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy RAB11B Ready-To-Use IHC Kit. RAB11B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28498-1-AP targets RAB11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RAB11B fusion protein Ag25429 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB11B, member RAS oncogene family
别名 Ras-related protein Rab-11B, Ras related protein Rab 11B, H YPT3, GTP-binding protein YPT3, GTP binding protein YPT3
计算分子量 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC110081
基因名称 RAB11B
Gene ID (NCBI) 9230
RRIDAB_2881156
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15907
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB11B antibody 28498-1-APDownload protocol
IHC protocol for RAB11B antibody 28498-1-APDownload protocol
IF protocol for RAB11B antibody 28498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pathol Res Pract

SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma

Authors - Chuandong Cheng
View Article