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RAB11B Polyclonal antibody

RAB11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28498-1-AP

Print datasheet

Synonyms

Ras-related protein Rab-11B, Ras related protein Rab 11B, H YPT3, GTP-binding protein YPT3, GTP binding protein YPT3

验证数据展示

  • WB analysis using 28498-1-AP

    WB analysis using 28498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28498-1-AP (RAB11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 28498-1-AP

    IF Staining of A549 using 28498-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11B antibody (28498-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 28498-1-AP

    IHC staining of human stomach using 28498-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28498-1-AP (RAB11B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, PC-12 cells, SH-SY5Y cells, RAW 264.7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, NIH3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28498-1-AP targets RAB11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RAB11B fusion protein Ag25429 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB11B, member RAS oncogene family
Synonyms Ras-related protein Rab-11B, Ras related protein Rab 11B, H YPT3, GTP-binding protein YPT3, GTP binding protein YPT3
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC110081
Gene Symbol RAB11B
Gene ID (NCBI) 9230
RRIDAB_2881156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB11B antibody 28498-1-APDownload protocol
IHC protocol for RAB11B antibody 28498-1-APDownload protocol
IF protocol for RAB11B antibody 28498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pathol Res Pract

SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma

Authors - Chuandong Cheng
View Article