验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HEK-293 cells, C6 cells, PC-12 cells, fetal human brain tissue, human testis tissue, mouse testis tissue, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lung tissue, human brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAB11B Ready-To-Use IHC Kit. RAB11B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19742-1-AP targets RAB11B-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, canine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB11B, member RAS oncogene family |
| 别名 | GTP binding protein YPT3, H YPT3, RAB11B, RAB11B-Specific, Ras related protein Rab 11B, YPT3 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | NM_004218 |
| 基因名称 | RAB11B |
| Gene ID (NCBI) | 9230 |
| RRID | AB_10642010 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15907 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB11B-Specific antibody 19742-1-AP | Download protocol |
| IHC protocol for RAB11B-Specific antibody 19742-1-AP | Download protocol |
| IP protocol for RAB11B-Specific antibody 19742-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. | ||
J Exp Clin Cancer Res Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway. | ||
Tumour Biol AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. | ||
J Histochem Cytochem Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties. | ||
J Neurosci The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2. | ||
Heliyon Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9 |
