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  • Featured Product
  • KD/KO Validated

RAB11FIP3 Polyclonal antibody

RAB11FIP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25843-1-AP

Print datasheet

Synonyms

ARFO1, Arfophilin 1, Eferin, FIP3 Rab11, KIAA0665, MU MB 17.148, Rab11 FIP3, RAB11FIP3

验证数据展示

  • IHC staining of human kidney using 25843-1-AP

    IHC staining of human kidney using 25843-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25843-1-AP (RAB11FIP3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 25843-1-AP

    IHC staining of human kidney using 25843-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25843-1-AP (RAB11FIP3 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of rat brain using 25843-1-AP

    WB analysis of rat brain using 25843-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25843-1-AP (RAB11FIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25843-1-AP is RAB11FIP3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RAB11FIP3 fusion protein Ag22937 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB11 family interacting protein 3 (class II)
Synonyms ARFO1, Arfophilin 1, Eferin, FIP3 Rab11, KIAA0665, MU MB 17.148, Rab11 FIP3, RAB11FIP3
Calculated Molecular Weight 756 aa, 82 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC051360
Gene Symbol RAB11FIP3
Gene ID (NCBI) 9727
RRIDAB_2880264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75154
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB11FIP3 antibody 25843-1-APDownload protocol
IHC protocol for RAB11FIP3 antibody 25843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Dev Cell

Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.

Authors - Vijay Walia
  • KD Validated
View Article
humanIF

J Cell Sci

Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

Authors - Nicholas V G Iannantuono
View Article
humanIF

J Cell Sci

Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

Authors - Nicholas V G Iannantuono
View Article