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  • Featured Product
  • KD/KO Validated

RAB11FIP5 Polyclonal antibody

RAB11FIP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14594-1-AP

Print datasheet

Synonyms

RFIP5, Rab11-interacting protein Rip11, Rab11 family-interacting protein 5, Gamma-SNAP-associated factor 1, Gaf-1

验证数据展示

  • WB analysis using 14594-1-AP

    WB analysis using 14594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 14594-1-AP

    IHC staining of human cervical cancer using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB11FIP5 antibody included
  • IHC staining of human cervical cancer using 14594-1-AP

    IHC staining of human cervical cancer using 14594-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB11FIP5 antibody included

经过测试的应用

Positive WB detected inA431 cells, mouse kidney tissue, mouse lung tissue, HeLa cells, mouse heart tissue, HEK-293T cells, rat heart tissue
Positive IHC detected inhuman cervical cancer tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB11FIP5 Ready-To-Use IHC Kit. RAB11FIP5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14594-1-AP targets RAB11FIP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RAB11FIP5 fusion protein Ag6128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB11 family interacting protein 5 (class I)
Synonyms RFIP5, Rab11-interacting protein Rip11, Rab11 family-interacting protein 5, Gamma-SNAP-associated factor 1, Gaf-1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC035013
Gene Symbol RAB11FIP5
Gene ID (NCBI) 26056
RRIDAB_10860101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.

实验方案

Product Specific Protocols
WB protocol for RAB11FIP5 antibody 14594-1-APDownload protocol
IHC protocol for RAB11FIP5 antibody 14594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Authors - Kai He
  • KD Validated
View Article