验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse kidney tissue, mouse lung tissue, HeLa cells, mouse heart tissue, HEK-293T cells, rat heart tissue |
| Positive IHC detected in | human cervical cancer tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAB11FIP5 Ready-To-Use IHC Kit. RAB11FIP5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14594-1-AP targets RAB11FIP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | RAB11FIP5 fusion protein Ag6128 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB11 family interacting protein 5 (class I) |
| 别名 | GAF1, Gaf-1, Gamma-SNAP-associated factor 1, Rab11 family-interacting protein 5, Rab11-interacting protein Rip11 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC035013 |
| 基因名称 | RAB11FIP5 |
| Gene ID (NCBI) | 26056 |
| RRID | AB_10860101 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB11FIP5 antibody 14594-1-AP | Download protocol |
| IHC protocol for RAB11FIP5 antibody 14594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
