验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18843-1-AP targets RAB12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB12, member RAS oncogene family |
| 别名 | Ras-related protein Rab-12, Ras related protein Rab 12 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | NM_001025300 |
| 基因名称 | RAB12 |
| Gene ID (NCBI) | 201475 |
| RRID | AB_10603469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6IQ22 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB12 antibody 18843-1-AP | Download protocol |
| IHC protocol for RAB12 antibody 18843-1-AP | Download protocol |
| IF protocol for RAB12 antibody 18843-1-AP | Download protocol |
| IP protocol for RAB12 antibody 18843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes. | ||
EMBO Rep Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy. | ||
Neurobiol Dis Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment. | ||
J Immunol Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.
| ||
Front Oncol Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest.
| ||
Traffic Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.
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