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RAB12 Polyclonal antibody

RAB12 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 29927-1-AP

Print datasheet

Synonyms

Ras-related protein Rab-12, Ras related protein Rab 12

验证数据展示

  • WB analysis using 29927-1-AP

    WB analysis using 29927-1-AP

    Human brain tissue was subjected to SDS PAGE followed by western blot with 29927-1-AP (RAB12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 29927-1-AP

    IP experiment of mouse brain using 29927-1-AP

    IP result of anti-RAB12 (IP:29927-1-AP, 4ug; Detection:29927-1-AP 1:1500) with mouse brain tissue lysate 2100 ug.

经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29927-1-AP targets RAB12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB12 fusion protein Ag31627 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB12, member RAS oncogene family
Synonyms Ras-related protein Rab-12, Ras related protein Rab 12
Calculated Molecular Weight27KD
Observed Molecular Weight 27 kDa
GenBank Accession NumberNM_001025300
Gene Symbol RAB12
Gene ID (NCBI) 201475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IQ22
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab12, a previously uncharacterized Rab isoform, is mainly localized at TfR-positive recycling endosomes and is specifically involved in TfR degradation. Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes.

实验方案

Product Specific Protocols
WB protocol for RAB12 antibody 29927-1-APDownload protocol
IP protocol for RAB12 antibody 29927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols