验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, HepG2 cells, Raji cells, human brain tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAB14 Ready-To-Use IHC Kit. RAB14 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15662-1-AP targets RAB14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | RAB14 fusion protein Ag8092 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB14, member RAS oncogene family |
| 别名 | FBP, RAB 14, RAB14, Ras related protein Rab 14 |
| 计算分子量 | 215 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC006081 |
| 基因名称 | RAB14 |
| Gene ID (NCBI) | 51552 |
| RRID | AB_2173630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61106 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB14 antibody 15662-1-AP | Download protocol |
| IHC protocol for RAB14 antibody 15662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Physiol Biochem Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer. | ||
Onco Targets Ther Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers. | ||
PLoS One Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway. | ||
Tumour Biol Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway.
| ||
Eur Rev Med Pharmacol Sci STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14. |
