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验证数据展示

  • IHC staining of mouse ovary using 67953-1-Ig

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB14 antibody included
  • WB analysis using 67953-1-Ig

    WB analysis using 67953-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of pig brain using 67953-1-Ig

    WB analysis of pig brain using 67953-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67953-1-Ig

    IHC staining of human colon cancer using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB14 antibody included
  • IHC staining of mouse ovary using 67953-1-Ig

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB14 antibody included
  • IF Staining of HeLa using 67953-1-Ig

    IF Staining of HeLa using 67953-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB14 antibody (67953-1-Ig, Clone: 3G7B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

RAB14 Monoclonal antibody

RAB14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67953-1-Ig
CloneNo. 3G7B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ras-related protein Rab-14, Ras related protein Rab 14, RAB 14, FBP, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, pig brain tissue, HCT 116 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse ovary tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB14 Ready-To-Use IHC Kit. RAB14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67953-1-Ig targets RAB14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen RAB14 fusion protein Ag8167 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB14, member RAS oncogene family
Synonyms Ras-related protein Rab-14, Ras related protein Rab 14, RAB 14, FBP, EC:3.6.5.2
Calculated Molecular Weight 215 aa, 24 kDa
GenBank Accession NumberBC006081
Gene Symbol RAB14
Gene ID (NCBI) 51552
RRIDAB_2918705
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB14 antibody 67953-1-IgDownload protocol
IHC protocol for RAB14 antibody 67953-1-IgDownload protocol
IF protocol for RAB14 antibody 67953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols