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RAB14 Monoclonal antibody

RAB14 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G7B3

Cat no : 67953-1-Ig

Print datasheet

Synonyms

FBP, RAB 14, RAB14, Ras related protein Rab 14

验证数据展示

  • IHC staining of mouse ovary using 67953-1-Ig

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB14 antibody included
  • WB analysis using 67953-1-Ig

    WB analysis using 67953-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of pig brain using 67953-1-Ig

    WB analysis of pig brain using 67953-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67953-1-Ig

    IHC staining of human colon cancer using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB14 antibody included
  • IHC staining of mouse ovary using 67953-1-Ig

    IHC staining of mouse ovary using 67953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB14 antibody included

经过测试的应用

Positive WB detected inLNCaP cells, pig brain tissue, HCT 116 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse ovary tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB14 Ready-To-Use IHC Kit. RAB14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67953-1-Ig targets RAB14 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen RAB14 fusion protein Ag8167 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB14, member RAS oncogene family
Synonyms FBP, RAB 14, RAB14, Ras related protein Rab 14
Calculated Molecular Weight 215 aa, 24 kDa
GenBank Accession NumberBC006081
Gene Symbol RAB14
Gene ID (NCBI) 51552
RRIDAB_2918705
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB14 antibody 67953-1-IgDownload protocol
IHC protocol for RAB14 antibody 67953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols