验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | WIF-B cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:25-1:50 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17501-1-AP targets RAB17 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RAB17 fusion protein Ag11688 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB17, member RAS oncogene family |
| 别名 | Ras-related protein Rab-17, Ras related protein Rab 17 |
| 计算分子量 | 212 aa, 23 kDa |
| GenBank蛋白编号 | BC050426 |
| 基因名称 | RAB17 |
| Gene ID (NCBI) | 64284 |
| RRID | AB_2878413 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0T7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB17 is a member of the RAS oncogene family and is classified as a small GTPase that plays a crucial role in intracellular membrane trafficking It is specifically expressed in epithelial cells and is involved in various cellular functions such as transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is important for the transcellular transport of substances like immunoglobulins from the basolateral surface to the apical surface. RAB17 is also implicated in the regulation of melanosome transport and release from melanocytes, which is critical for pigmentation in skin cells . Additionally, it has been suggested to regulate dendrite and dendritic spine development, which are important for neuronal function. The protein is involved in membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB17 antibody 17501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer.
| ||
Cell Death Dis RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis
| ||
Int J Mol Sci The Tolerogenic Influence of Dexamethasone on Dendritic Cells Is Accompanied by the Induction of Efferocytosis, Promoted by MERTK | ||
Cell Death Dis Rab17 mediates differential antigen sorting following efferocytosis and phagocytosis. | ||
J Biol Chem The GTP-bound and Sumoylated Form of the rab17 Small Molecular Weight GTPase Selectively Binds Syntaxin 2 in Polarized Hepatic WIF-B Cells. | ||