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验证数据展示

  • WB analysis of fetal human brain using 28274-1-AP

    WB analysis of fetal human brain using 28274-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28274-1-AP (RAB17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 28274-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28274-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28274-1-AP (RAB17 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 28274-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28274-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28274-1-AP (RAB17 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 28274-1-AP

    IF Staining of MCF-7 using 28274-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 28274-1-AP (RAB17 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAB17 Polyclonal antibody

RAB17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28274-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ras-related protein Rab-17, Ras related protein Rab 17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28274-1-AP targets RAB17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen RAB17 fusion protein Ag27206 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB17, member RAS oncogene family
Synonyms Ras-related protein Rab-17, Ras related protein Rab 17
Calculated Molecular Weight 212 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC050426
Gene Symbol RAB17
Gene ID (NCBI) 64284
RRIDAB_2881103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0T7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB17 is a member of the RAS oncogene family and is classified as a small GTPase that plays a crucial role in intracellular membrane trafficking It is specifically expressed in epithelial cells and is involved in various cellular functions such as transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is important for the transcellular transport of substances like immunoglobulins from the basolateral surface to the apical surface. RAB17 is also implicated in the regulation of melanosome transport and release from melanocytes, which is critical for pigmentation in skin cells. Additionally, it has been suggested to regulate dendrite and dendritic spine development, which are important for neuronal function. The protein is involved in membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis.

实验方案

Product Specific Protocols
WB protocol for RAB17 antibody 28274-1-APDownload protocol
IHC protocol for RAB17 antibody 28274-1-APDownload protocol
IF protocol for RAB17 antibody 28274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols