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RAB18 Monoclonal antibody

RAB18 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2E4B3

Cat no : 67659-1-Ig

Print datasheet

Synonyms

RAB18, RAB18LI1, Ras related protein Rab 18

验证数据展示

  • WB analysis using 67659-1-Ig

    WB analysis using 67659-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67659-1-Ig (RAB18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67659-1-Ig

    IHC staining of mouse brain using 67659-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67659-1-Ig (RAB18 antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67659-1-Ig

    IHC staining of mouse brain using 67659-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67659-1-Ig (RAB18 antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, human testis tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:175-1:700
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67659-1-Ig targets RAB18 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human, mouse, rat, pig
Cited Reactivitymouse
Immunogen RAB18 fusion protein Ag16669 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB18, member RAS oncogene family
Synonyms RAB18, RAB18LI1, Ras related protein Rab 18
Calculated Molecular Weight 206 aa, 23 kDa
GenBank Accession NumberBC015014
Gene Symbol RAB18
Gene ID (NCBI) 22931
RRIDAB_2882856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NP72
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB18 antibody 67659-1-IgDownload protocol
IHC protocol for RAB18 antibody 67659-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Gastroenterol Hepatol

Effect of Rab18 on liver injury and lipid accumulation by regulating perilipin 2 and peroxisome proliferator-activated receptor gamma in non-alcoholic fatty liver disease

Authors - Lei Zhang
View Article