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  • Featured Product
  • KD/KO Validated

RAB1B Polyclonal antibody

RAB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 17824-1-AP

Print datasheet

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2

验证数据展示

  • WB analysis using 17824-1-AP

    WB analysis using 17824-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17824-1-AP

    WB analysis of HeLa using 17824-1-AP

    WB result of RAB1B antibody (17824-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

  • WB analysis of HeLa using 17824-1-PBS

    WB analysis of HeLa using 17824-1-PBS

    HeLa cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 17824-1-AP

    IHC staining of human heart using 17824-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human heart using 17824-1-AP

    IHC staining of human heart using 17824-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human ovary tumor using 17824-1-AP

    IHC staining of human ovary tumor using 17824-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human ovary tumor using 17824-1-AP

    IHC staining of human ovary tumor using 17824-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IF Staining of NIH/3T3 using 17824-1-AP

    IF Staining of NIH/3T3 using 17824-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 17824-1-AP

    IF Staining of HeLa using 17824-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB1B antibody (17824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, HeLa cells, HAP1, A431 cells, NIH/3T3 cells
Positive IHC detected inhuman heart tissue, mouse heart tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17824-1-AP targets RAB1B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Immunogen RAB1B fusion protein Ag12197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB1B, member RAS oncogene family
Synonyms Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
RRIDAB_2237881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport,  was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes  that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).

实验方案

Product Specific Protocols
WB protocol for RAB1B antibody 17824-1-APDownload protocol
IHC protocol for RAB1B antibody 17824-1-APDownload protocol
IF protocol for RAB1B antibody 17824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

Authors - Janice D Thomas
View Article
humanWB,IF

Nat Cell Biol

A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion

Authors - Yuxin Sun
  • KD Validated
View Article
humanWB

Nat Cell Biol

A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

Authors - Reiling Jan H JH
View Article
humanIF

Dev Cell

SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.

Authors - Rui Yan
View Article
humanIHC

Aging (Albany NY)

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.

Authors - Xian-Zi Yang
View Article
ratWB

Front Pharmacol

Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.

Authors - Dong Deng
View Article