• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RAB21 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-80 ng/mL

Sandwich ELISA: 39.1-2500 pg/mL

Cat no : MP01552-1

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01552-1, Human RAB21 Recombinant Matched Antibody Pair - PBS only. 84776-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35291. 84776-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP01552-1

    Sandwich ELISA standard curve of MP01552-1, Human RAB21 Recombinant Matched Antibody Pair - PBS only. 84776-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35291. 84776-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • The mean RAB21 concentration was determined to be 10.3 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load, 9.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load and 5.5 ng/mL in HepG2 cell extract based on a 2.3 mg/mL extract load.

    Sample test of MP01552-1

    The mean RAB21 concentration was determined to be 10.3 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load, 9.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load and 5.5 ng/mL in HepG2 cell extract based on a 2.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP01552-1, RAB21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84776-3-PBS. Detection antibody: 84776-4-PBS. Standard: Ag35291. Range: 0.625-80 ng/mL.

    Cytometric bead array standard curve of MP01552-1

    Cytometric bead array standard curve of MP01552-1, RAB21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84776-3-PBS. Detection antibody: 84776-4-PBS. Standard: Ag35291. Range: 0.625-80 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01552-1 targets RAB21 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-80 ng/mL (Cytometric Bead Array); 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: RAB21 Recombinant antibody, PBS Only (Capture) 84776-3-PBS (242117C6). 100 μg. Concentration 1 mg/ml.

Detection antibody: RAB21 Recombinant antibody, PBS Only (Detector) 84776-4-PBS (242117E9). 100 μg. Concentration 1 mg/ml.

Alternative RAB21 matched antibody pairs: MP01552-2

Matched pair protein standard: Ag35291

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84776-3-PBS
Tested Reactivity human
Immunogen RAB21 fusion protein Ag35291
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB21, member RAS oncogene family
Synonyms Ras related protein Rab 21, KIAA0118, 242117C6
GenBank accession numberNM_014999
Gene symbol RAB21
UniprotQ9UL25
Gene ID (NCBI) 23011
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84776-4-PBS
Tested Reactivity human
Immunogen RAB21 fusion protein Ag35291
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB21, member RAS oncogene family
Synonyms Ras related protein Rab 21, KIAA0118, 242117E9
GenBank accession numberNM_014999
Gene symbol RAB21
UniprotQ9UL25
Gene ID (NCBI) 23011
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.