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  • Featured Product
  • KD/KO Validated

RAB27A Polyclonal antibody, PBS Only

RAB27A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17817-1-PBS

Print datasheet

Synonyms

Rab-27, RAB27, Rab 27, GTP-binding protein Ram, GTP binding protein Ram

验证数据展示

  • WB analysis using 17817-1-AP (same clone as 17817-1-PBS)

    WB analysis using 17817-1-AP (same clone as 17817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • WB analysis of K562 using 17817-1-AP (same clone as 17817-1-PBS)

    WB analysis of K562 using 17817-1-AP (same clone as 17817-1-PBS)

    WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

    IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

    IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

    IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

    IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • IP experiment of K-562 using 17817-1-AP (same clone as 17817-1-PBS)

    IP experiment of K-562 using 17817-1-AP (same clone as 17817-1-PBS)

    IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.

产品信息

17817-1-PBS targets RAB27A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RAB27A fusion protein Ag12136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB27A, member RAS oncogene family
Synonyms Rab-27, RAB27, Rab 27, GTP-binding protein Ram, GTP binding protein Ram
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC107680
Gene Symbol RAB27A
Gene ID (NCBI) 5873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51159
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.