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RAB27A Polyclonal antibody

RAB27A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16868-1-AP

Print datasheet

Synonyms

RAB27A-specific, Rab-27, RAB27, Rab 27, GTP-binding protein Ram

验证数据展示

  • WB analysis using 16868-1-AP

    WB analysis using 16868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of K562 using 16868-1-AP

    WB analysis of K562 using 16868-1-AP

    WB result of RAB27A antibody (16868-1-AP, 1:1000) with si-Control and si-RAB27A transfected K562 cells.

  • IHC staining of human lymphoma using 16868-1-AP

    IHC staining of human lymphoma using 16868-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human lymphoma using 16868-1-AP

    IHC staining of human lymphoma using 16868-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB27A antibody included

经过测试的应用

Positive WB detected inHL-60 cells, K562, PC-12 cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16868-1-AP targets RAB27A in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB27A, member RAS oncogene family
Synonyms RAB27A-specific, Rab-27, RAB27, Rab 27, GTP-binding protein Ram
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_004580
Gene Symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_2176716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

实验方案

Product Specific Protocols
WB protocol for RAB27A antibody 16868-1-APDownload protocol
IHC protocol for RAB27A antibody 16868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Involvement of Rab11 in osteoblastic differentiation: Its up-regulation during the differentiation and by tensile stress

Authors - Lay Thant
View Article