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验证数据展示

  • IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB32 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB32 antibody included
  • IF Staining of U-251 using 82960-5-RR (same clone as 82960-5-PBS)

    IF Staining of U-251 using 82960-5-RR (same clone as 82960-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82960-5-RR (same clone as 82960-5-PBS)

    FC experiment of HeLa using 82960-5-RR (same clone as 82960-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB32 Recombinant antibody (82960-5-RR, Clone:230315B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82960-5-RR

    Affinity and Kinetic Characterization of 82960-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82960-5-RR against Human RAB32 were performed. The affinity constant is 0.499 nM.

RAB32 Recombinant antibody, PBS Only

RAB32 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82960-5-PBS

产品说明书

CloneNo. 230315B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.

产品信息

82960-5-PBS targets RAB32 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 RAB32 fusion protein Ag1450 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB32, member RAS oncogene family
别名 Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10
计算分子量 25 kDa
GenBank蛋白编号BC015061
基因名称 RAB32
Gene ID (NCBI) 10981
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.