验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, U2OS cells, A549 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27435-1-AP targets RAB34 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | RAB34 fusion protein Ag26696 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB34, member RAS oncogene family |
| 别名 | Ras-related protein Rab-34, Ras related protein Rah, Ras related protein Rab 39, Ras related protein Rab 34, RAH |
| 计算分子量 | 251 aa, 28 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC016841 |
| 基因名称 | RAB34 |
| Gene ID (NCBI) | 83871 |
| RRID | AB_2880870 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB34, a Rab protein family member, is involved in repositioning of lysosomes, activation of micropinocytosis, and protein transport. RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. Recently, Rab34 is identified as a ciliary protein. Rab34 is required for internal assembly of cilia but not for cilia built on the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB34 antibody 27435-1-AP | Download protocol |
| IHC protocol for RAB34 antibody 27435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.
| ||
Biomedicines Signaling Switching from Hedgehog-GLI to MAPK Signaling Potentially Serves as a Compensatory Mechanism in Melanoma Cell Lines Resistant to GANT-61 | ||
Hum Mol Genet Pathogenic RAB34 variants impair primary cilium assembly and cause a novel oral-facial-digital syndrome | ||
bioRxiv A hierarchical pathway for assembly of the distal appendages that organize primary cilia | ||
bioRxiv Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages |
