验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy RAB35 Ready-To-Use IHC Kit. RAB35 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68552-1-Ig targets RAB35 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat, rabbit |
| 免疫原 | RAB35 fusion protein Ag21424 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAB35, member RAS oncogene family |
| 别名 | GTP binding protein RAY, H ray, RAB1C, RAB35, Ras related protein Rab 1C, Ras related protein Rab 35, RAY |
| 计算分子量 | 201 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC015931 |
| 基因名称 | RAB35 |
| Gene ID (NCBI) | 11021 |
| RRID | AB_3085256 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15286 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB35 antibody 68552-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |