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RAB38 Polyclonal antibody

RAB38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12234-1-AP

Print datasheet

Synonyms

Ras-related protein Rab-38, Ras related protein Rab 38, RAB 38, Melanoma antigen NY MEL 1

验证数据展示

  • WB analysis of NIH/3T3 using 12234-1-AP

    WB analysis of NIH/3T3 using 12234-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 12234-1-AP

    IF Staining of A549 using 12234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12234-1-AP (RAB38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, A375 cells
Positive IHC detected inhuman gliomas tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12234-1-AP targets RAB38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RAB38 fusion protein Ag2874 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB38, member RAS oncogene family
Synonyms Ras-related protein Rab-38, Ras related protein Rab 38, RAB 38, Melanoma antigen NY MEL 1
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC015808
Gene Symbol RAB38
Gene ID (NCBI) 23682
RRIDAB_2877839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB38 antibody 12234-1-APDownload protocol
IHC protocol for RAB38 antibody 12234-1-APDownload protocol
IF protocol for RAB38 antibody 12234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.

Authors - Saurabh Shakya
View Article
mouseIF

JBMR Plus

Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets

Authors - Yasunori Omata
View Article