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验证数据展示

  • WB analysis of NIH/3T3 using 12234-1-AP

    WB analysis of NIH/3T3 using 12234-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 12234-1-AP

    IF Staining of A549 using 12234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12234-1-AP (RAB38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RAB38 Polyclonal antibody

RAB38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12234-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Ras-related protein Rab-38, Ras related protein Rab 38, RAB 38, Melanoma antigen NY MEL 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A375 cells
Positive IHC detected inhuman gliomas tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12234-1-AP targets RAB38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 RAB38 fusion protein Ag2874 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB38, member RAS oncogene family
别名 Ras-related protein Rab-38, Ras related protein Rab 38, RAB 38, Melanoma antigen NY MEL 1
计算分子量 211 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC015808
基因名称 RAB38
Gene ID (NCBI) 23682
RRIDAB_2877839
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB38 antibody 12234-1-APDownload protocol
IHC protocol for RAB38 antibody 12234-1-APDownload protocol
IF protocol for RAB38 antibody 12234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.

Authors - Saurabh Shakya
View Article
mouseIF

JBMR Plus

Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets

Authors - Yasunori Omata
View Article