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验证数据展示

  • WB analysis of mouse brain using 16865-1-AP

    WB analysis of mouse brain using 16865-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16865-1-AP (RAB3A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 16865-1-AP

    IHC staining of human pancreas using 16865-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16865-1-AP (RAB3A-specific Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RAB3A antibody included
  • IHC staining of human pancreas using 16865-1-AP

    IHC staining of human pancreas using 16865-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16865-1-AP (RAB3A-specific Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RAB3A antibody included
  • IF Staining of HeLa using 16865-1-AP

    IF Staining of HeLa using 16865-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB3A-specific antibody (16865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RAB3A-specific Polyclonal antibody

RAB3A-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16865-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

RAB3A, Ras-related protein Rab-3A, Ras related protein Rab 3A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB3A Ready-To-Use IHC Kit. RAB3A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16865-1-AP targets RAB3A-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3A, member RAS oncogene family
别名 RAB3A, Ras-related protein Rab-3A, Ras related protein Rab 3A
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号NM_002866
基因名称 RAB3A
Gene ID (NCBI) 5864
RRIDAB_11182389
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20336
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab3 subfamily includes Rab3A, Rab3B, Rab3C, and Rab3D, involving in the exocytosis of neurotransmitters and hormones. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. This antibody is specific to Rab3A.

实验方案

Product Specific Protocols
WB protocol for RAB3A-specific antibody 16865-1-APDownload protocol
IHC protocol for RAB3A-specific antibody 16865-1-APDownload protocol
IF protocol for RAB3A-specific antibody 16865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols