验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16865-1-AP targets RAB3A-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RAB3A, member RAS oncogene family |
Synonyms | RAB3A, Ras-related protein Rab-3A, Ras related protein Rab 3A |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | NM_002866 |
Gene Symbol | RAB3A |
Gene ID (NCBI) | 5864 |
RRID | AB_11182389 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20336 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Rab3 subfamily includes Rab3A, Rab3B, Rab3C, and Rab3D, involving in the exocytosis of neurotransmitters and hormones. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. This antibody is specific to Rab3A.
实验方案
Product Specific Protocols | |
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WB protocol for RAB3A-specific antibody 16865-1-AP | Download protocol |
IHC protocol for RAB3A-specific antibody 16865-1-AP | Download protocol |
IF protocol for RAB3A-specific antibody 16865-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |