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RAB3A-specific Polyclonal antibody

RAB3A-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16865-1-AP

Print datasheet

Synonyms

RAB3A, Ras-related protein Rab-3A, Ras related protein Rab 3A

验证数据展示

  • WB analysis of mouse brain using 16865-1-AP

    WB analysis of mouse brain using 16865-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16865-1-AP (RAB3A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 16865-1-AP

    IHC staining of human pancreas using 16865-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16865-1-AP (RAB3A-specific Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RAB3A antibody included
  • IHC staining of human pancreas using 16865-1-AP

    IHC staining of human pancreas using 16865-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16865-1-AP (RAB3A-specific Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RAB3A antibody included
  • IF Staining of HeLa using 16865-1-AP

    IF Staining of HeLa using 16865-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB3A-specific antibody (16865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB3A Ready-To-Use IHC Kit. RAB3A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16865-1-AP targets RAB3A-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB3A, member RAS oncogene family
Synonyms RAB3A, Ras-related protein Rab-3A, Ras related protein Rab 3A
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_002866
Gene Symbol RAB3A
Gene ID (NCBI) 5864
RRIDAB_11182389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20336
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab3 subfamily includes Rab3A, Rab3B, Rab3C, and Rab3D, involving in the exocytosis of neurotransmitters and hormones. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. This antibody is specific to Rab3A.

实验方案

Product Specific Protocols
WB protocol for RAB3A-specific antibody 16865-1-APDownload protocol
IHC protocol for RAB3A-specific antibody 16865-1-APDownload protocol
IF protocol for RAB3A-specific antibody 16865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols