验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HL-60 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21663-1-AP targets RAB3GAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | RAB3GAP1 fusion protein Ag16426 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB3 GTPase activating protein subunit 1 (catalytic) |
| 别名 | KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3 GTPase-activating protein 1, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1 |
| 计算分子量 | 981 aa, 111 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC146809 |
| 基因名称 | RAB3GAP1 |
| Gene ID (NCBI) | 22930 |
| RRID | AB_10863124 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15042 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB3GAP1 (RAB3 GTPase-activating protein 1) is the catalytic subunit of the heterodimeric enzyme RAB3GAP (RAB3GTPase-activating protein), a key regulator of the RAB3 cycle, which controls calcium-mediated exocytosis of neurotransmitters and hormones. Mutations in RAB3GAP1 cause Warburg Micro syndrome (OMIM 600118) and Martsolf syndrome (OMIM 212720), phenotypically overlapping autosomal recessive conditions characterized by ocular and neurodevelopmental manifestations.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| IHC protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| IF protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Rab18 and a Rab18 GEF complex are required for normal ER structure.
| ||
BMC Biol VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase. | ||
J Neurosci Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP. | ||
Toxicol Lett Manganese-induced alpha-synuclein overexpression impairs synaptic vesicle fusion by disrupting the Rab3 cycle in primary cultured neurons. | ||