验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse brain tissue, rat brain tissue, HeLa cells, Neuro-2a cells, PC-12 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
10347-1-AP targets RAB4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RAB4A fusion protein Ag0357 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB4A, member RAS oncogene family |
| 别名 | Ras-related protein Rab-4A, Ras related protein Rab 4A, RAB4, HRES 1/RAB4, EC:3.6.5.2 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 24-28 kDa |
| GenBank蛋白编号 | BC002438 |
| 基因名称 | RAB4A |
| Gene ID (NCBI) | 5867 |
| RRID | AB_2177544 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20338 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human RAB genes share structural and biochemical properties with the Ras gene superfamily, and RAB4 (synonym: RAB4A) is a small GTPase belonging to this Ras superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. RAB4 may also function as regulator along the receptor recycling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB4A antibody 10347-1-AP | Download protocol |
| IHC protocol for RAB4A antibody 10347-1-AP | Download protocol |
| IF protocol for RAB4A antibody 10347-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
MBio Yersinia pestisTargets the Host Endosome Recycling Pathway during the Biogenesis of theYersinia-Containing Vacuole To Avoid Killing by Macrophages. | ||
Virology Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells. | ||
Pathog Dis Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages. | ||
FASEB J Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R. |
