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Species Reactivity
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Species Reactivity
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Conjugate
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0
Conjugate
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验证数据展示

  • WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

    WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

    IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

    IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

RAB5A Monoclonal antibody, PBS Only

RAB5A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66339-1-PBS

产品说明书

CloneNo. 1B6A5

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RAB5, RAB5A, Ras related protein Rab 5A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66339-1-PBS targets RAB5A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 RAB5A fusion protein Ag16781 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB5A, member RAS oncogene family
别名 RAB5, RAB5A, Ras related protein Rab 5A
计算分子量 215 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC001267
基因名称 RAB5A
Gene ID (NCBI) 5868
RRIDAB_2881719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20339
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.