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RAB5A Monoclonal antibody, PBS Only

RAB5A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6A5

Cat no : 66339-1-PBS

Print datasheet

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

验证数据展示

  • WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

    WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

    IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

    IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

产品信息

66339-1-PBS targets RAB5A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen RAB5A fusion protein Ag16781 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RAB5A, member RAS oncogene family
Synonyms RAB5, RAB5A, Ras related protein Rab 5A
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20339
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.