验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells |
Positive IF/ICC detected in | NIH/3T3 cells, MDCK cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30636-1-AP targets RAB8A in WB, IF/ICC, ELISA applications and shows reactivity with Human, canine, mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, canine, mouse |
免疫原 | RAB8A fusion protein Ag30567 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAB8A, member RAS oncogene family |
别名 | MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A |
计算分子量 | 24 kDa |
观测分子量 | 24 kDa |
GenBank蛋白编号 | NM_005370 |
基因名称 | RAB8A |
Gene ID (NCBI) | 4218 |
RRID | AB_3086378 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P61006 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.
实验方案
Product Specific Protocols | |
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WB protocol for RAB8A antibody 30636-1-AP | Download protocol |
IF protocol for RAB8A antibody 30636-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |