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验证数据展示

  • WB analysis using 30636-1-AP

    WB analysis using 30636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30636-1-AP (RAB8A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MDCK using 30636-1-AP

    IF Staining of MDCK using 30636-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 30636-1-AP

    IF Staining of NIH/3T3 using 30636-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

RAB8A Polyclonal antibody

RAB8A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30636-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, canine, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inNIH/3T3 cells, MDCK cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30636-1-AP targets RAB8A in WB, IF/ICC, ELISA applications and shows reactivity with Human, canine, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, canine, mouse
Immunogen RAB8A fusion protein Ag30567 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB8A, member RAS oncogene family
Synonyms MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberNM_005370
Gene Symbol RAB8A
Gene ID (NCBI) 4218
RRIDAB_3086378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.

实验方案

Product Specific Protocols
WB protocol for RAB8A antibody 30636-1-APDownload protocol
IF protocol for RAB8A antibody 30636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols