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RABAC1 Polyclonal antibody

RABAC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10542-1-AP

Print datasheet

Synonyms

Prenylated Rab acceptor protein 1, PRAF1, PRA1 family protein 1, PRA1

验证数据展示

  • WB analysis of HEK-293 using 10542-1-AP

    WB analysis of HEK-293 using 10542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10542-1-AP

    IHC staining of human pancreas cancer using 10542-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HEK-293 using 10542-1-AP

    IF Staining of HEK-293 using 10542-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, human pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10542-1-AP targets RABAC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RABAC1 fusion protein Ag0830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rab acceptor 1 (prenylated)
Synonyms Prenylated Rab acceptor protein 1, PRAF1, PRA1 family protein 1, PRA1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC008950
Gene Symbol RABAC1
Gene ID (NCBI) 10567
RRIDAB_2175639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI14
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RABAC1 antibody 10542-1-APDownload protocol
IHC protocol for RABAC1 antibody 10542-1-APDownload protocol
IF protocol for RABAC1 antibody 10542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

BMC Neurosci

A role for prenylated rab acceptor 1 in vertebrate photoreceptor development.

Authors - Dickison Virginia M VM
View Article
mouse,humanWB,IF

PLoS One

Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites.

Authors - Ameair Abu Irqeba
View Article
mouseWB

Biochim Biophys Acta Proteins Proteom

Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.

Authors - Uchenna Emechebe
View Article