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验证数据展示

  • WB analysis of HEK-293 using 10542-1-AP

    WB analysis of HEK-293 using 10542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10542-1-AP

    IHC staining of human pancreas cancer using 10542-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HEK-293 using 10542-1-AP

    IF Staining of HEK-293 using 10542-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RABAC1 Polyclonal antibody

RABAC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10542-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Prenylated Rab acceptor protein 1, PRAF1, PRA1 family protein 1, PRA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, human pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10542-1-AP targets RABAC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RABAC1 fusion protein Ag0830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rab acceptor 1 (prenylated)
别名 Prenylated Rab acceptor protein 1, PRAF1, PRA1 family protein 1, PRA1
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC008950
基因名称 RABAC1
Gene ID (NCBI) 10567
RRIDAB_2175639
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI14
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RABAC1 antibody 10542-1-APDownload protocol
IHC protocol for RABAC1 antibody 10542-1-APDownload protocol
IF protocol for RABAC1 antibody 10542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

BMC Neurosci

A role for prenylated rab acceptor 1 in vertebrate photoreceptor development.

Authors - Dickison Virginia M VM
View Article
mouse,humanWB,IF

PLoS One

Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites.

Authors - Ameair Abu Irqeba
View Article
mouseWB

Biochim Biophys Acta Proteins Proteom

Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.

Authors - Uchenna Emechebe
View Article