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验证数据展示

  • WB analysis of Jurkat using 60125-1-Ig

    WB analysis of Jurkat using 60125-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 60125-1-Ig

    WB analysis of HSC-T6 using 60125-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60125-1-Ig

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 60125-1-Ig

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

RABEP2 Monoclonal antibody

RABEP2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60125-1-Ig

产品说明书

CloneNo. 1A4A4

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, ELISA

别名

FRA, Rabaptin 5beta, RABEP2, RABPT5B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHSC-T6 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60125-1-Ig targets RABEP2 in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat, pig
免疫原 RABEP2 fusion protein Ag6197 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 rabaptin, RAB GTPase binding effector protein 2
别名 FRA, Rabaptin 5beta, RABEP2, RABPT5B
计算分子量 64 kDa
观测分子量 62 kDa
GenBank蛋白编号BC058900
基因名称 RABEP2
Gene ID (NCBI) 79874
RRIDAB_2284821
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H5N1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

实验方案

Product Specific Protocols
WB protocol for RABEP2 antibody 60125-1-IgDownload protocol
IHC protocol for RABEP2 antibody 60125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols