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验证数据展示

  • WB analysis of HepG2 using 10213-2-AP

    WB analysis of HepG2 using 10213-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung squamous cell carcinoma using 10213-2-AP

    IHC staining of human lung squamous cell carcinoma using 10213-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RABEPK antibody included

RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10213-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RABEPK Ready-To-Use IHC Kit. RABEPK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10213-2-AP targets RABEPK/p40 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RABEPK/p40 fusion protein Ag0285 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rab9 effector protein with kelch motifs
别名 p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC000503
基因名称 RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_2238057
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z6M1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

实验方案

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 10213-2-APDownload protocol
IHC protocol for RABEPK/p40 antibody 10213-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols