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验证数据展示

  • WB analysis using 14223-1-AP

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 14223-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 14223-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14223-1-AP (RABGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RABGAP1 Polyclonal antibody

RABGAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14223-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Rab6 GTPase-activating protein GAPCenA, Rab GTPase-activating protein 1, HSPC094, GAPCENA, GAP and centrosome-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14223-1-AP targets RABGAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RABGAP1 fusion protein Ag5462 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB GTPase activating protein 1
别名 Rab6 GTPase-activating protein GAPCenA, Rab GTPase-activating protein 1, HSPC094, GAPCENA, GAP and centrosome-associated protein
计算分子量 122 kDa
观测分子量 121 kDa
GenBank蛋白编号BC054492
基因名称 RABGAP1
Gene ID (NCBI) 23637
RRIDAB_2878030
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3P9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RABGAP1 antibody 14223-1-APDownload protocol
IHC protocol for RABGAP1 antibody 14223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols