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验证数据展示

  • WB analysis using 83698-4-RR

    WB analysis using 83698-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83698-4-RR (RABGAP1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83698-4-RR

    FC experiment of HeLa using 83698-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RABGAP1L Recombinant antibody (83698-4-RR, Clone:240663G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83698-4-RR

    Affinity and Kinetic Characterization of 83698-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83698-4-RR against Human RABGAP1L were performed. The affinity constant is below 1 pM.

RABGAP1L Recombinant monoclonal antibody

RABGAP1L Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83698-4-RR

产品说明书

CloneNo. 240663G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240663G12, Rab GTPase-activating protein 1-like, isoform 10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83698-4-RR targets RABGAP1L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4856

Product name: Recombinant human RABGAP1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC041888

Sequence: MEVRASLQKIVSNGDEQLEKAMEEILRDSEKRPSSLLVDCQSSSEISDHSFGDIPASQTNKPSLQLILDPSNTEISTPRPSSPGGLPEEDSVLFNKLTYLGCMKVSSPRNEVEALRAMATMKSSSQYPFPVTLYVPNVPEGSVRIIDQSSNVEIASFPIYKVLFCARGHDGTTESNCFAFTESSHGSEEFQIHVFSCEIKEAVSRILYSFCTAFKRSSRQVSDVKDSVIPTPDSDVFTFSVSLEVKEDDGKGNFSPVPKDRDKFYFKLKQGIEKKVVITVQQLSNKELAIERCFGMLLSPGRNVKNSDMHLLDMESMGKSYDGRAYVITGMWNPNAPVFLALNEETPKDKQVYMT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB GTPase activating protein 1-like
别名 240663G12, Rab GTPase-activating protein 1-like, isoform 10
计算分子量 815 aa, 93 kDa
观测分子量100 kDa,140 kDa
GenBank蛋白编号BC041888
基因名称 RABGAP1L
Gene ID (NCBI) 9910
RRIDAB_3671301
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDB7ZAP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.

实验方案

Product Specific Protocols
FC protocol for RABGAP1L antibody 83698-4-RRDownload protocol
WB protocol for RABGAP1L antibody 83698-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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