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RABIF Polyclonal antibody

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29113-1-AP

Print datasheet

Synonyms

MSS4, RAB interacting factor, RABIF, RASGFR3, RASGRF3

验证数据展示

  • IHC staining of mouse brain using 29113-1-AP

    IHC staining of mouse brain using 29113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29113-1-AP

    WB analysis using 29113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 29113-1-AP

    IF Staining of MCF-7 using 29113-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29113-1-AP targets RABIF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RABIF fusion protein Ag30761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB interacting factor
Synonyms MSS4, RAB interacting factor, RABIF, RASGFR3, RASGRF3
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC018488
Gene Symbol RABIF
Gene ID (NCBI) 5877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).

实验方案

Product Specific Protocols
WB protocol for RABIF antibody 29113-1-APDownload protocol
IHC protocol for RABIF antibody 29113-1-APDownload protocol
IF protocol for RABIF antibody 29113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols