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RABL2B Recombinant antibody

RABL2B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240501D3

Cat no : 83470-3-RR

Print datasheet

Synonyms

Rab-like protein 2B, Rab like protein 2B, 240501D3

验证数据展示

  • WB analysis using 83470-3-RR

    WB analysis using 83470-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83470-3-RR

    FC experiment of A549 using 83470-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of MCF-7 using 83470-3-RR

    FC experiment of MCF-7 using 83470-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83470-3-RR

    Affinity and Kinetic Characterization of 83470-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive FC (Intra) detected inA549 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83470-3-RR is RABL2B Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RABL2B fusion protein Ag2189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB, member of RAS oncogene family-like 2B
Synonyms Rab-like protein 2B, Rab like protein 2B, 240501D3
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC024281
Gene Symbol RABL2B
Gene ID (NCBI) 11158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UNT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).

实验方案

Product Specific Protocols
WB protocol for RABL2B antibody 83470-3-RRDownload protocol
FC protocol for RABL2B antibody 83470-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols