• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83470-3-RR

    WB analysis using 83470-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83470-3-RR

    FC experiment of A549 using 83470-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of MCF-7 using 83470-3-RR

    FC experiment of MCF-7 using 83470-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83470-3-RR

    Affinity and Kinetic Characterization of 83470-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

RABL2B Recombinant antibody

RABL2B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83470-3-RR

产品说明书

CloneNo. 240501D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Rab-like protein 2B, Rab like protein 2B, 240501D3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive FC (Intra) detected inA549 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83470-3-RR targets RABL2B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RABL2B fusion protein Ag2189 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB, member of RAS oncogene family-like 2B
别名 Rab-like protein 2B, Rab like protein 2B, 240501D3
计算分子量 229 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC024281
基因名称 RABL2B
Gene ID (NCBI) 11158
RRIDAB_3671100
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UNT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).

实验方案

Product Specific Protocols
WB protocol for RABL2B antibody 83470-3-RRDownload protocol
FC protocol for RABL2B antibody 83470-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols