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RABL3 Monoclonal antibody

RABL3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H3E1

Cat no : 68342-1-Ig

Print datasheet

Synonyms

Rab like protein 3, RABL3

验证数据展示

  • WB analysis using 68342-1-Ig

    WB analysis using 68342-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68342-1-Ig (RABL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of A549 using 68342-1-Ig

    IF Staining of A549 using 68342-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABL3 antibody (68342-1-Ig, Clone: 2H3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68342-1-Ig targets RABL3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen RABL3 fusion protein Ag33137 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB, member of RAS oncogene family-like 3
Synonyms Rab like protein 3, RABL3
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC020832
Gene Symbol RABL3
Gene ID (NCBI) 285282
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ5HYI8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

实验方案

Product Specific Protocols
WB protocol for RABL3 antibody 68342-1-IgDownload protocol
IF protocol for RABL3 antibody 68342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols