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- KD/KO Validated
RAC2 Polyclonal antibody
RAC2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HL-60 cells, mouse spleen tissue, rat spleen tissue |
Positive IP detected in | HL-60 cells |
Positive IHC detected in | human tonsillitis tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 7 publications below |
产品信息
10735-1-AP targets RAC2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | RAC2 fusion protein Ag1202 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) |
Synonyms | EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC001485 |
Gene Symbol | RAC2 |
Gene ID (NCBI) | 5880 |
RRID | AB_2176127 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P15153 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RAC2 antibody 10735-1-AP | Download protocol |
IHC protocol for RAC2 antibody 10735-1-AP | Download protocol |
IP protocol for RAC2 antibody 10735-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Genet In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression.
| ||
J Exp Clin Cancer Res Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway. | ||
Front Mol Neurosci Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis. | ||
Oncol Rep Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. | ||
Biochem Biophys Res Commun Hsa_circ_001659 serves as a novel diagnostic and prognostic biomarker for colorectal cancer. | ||
Tumour Biol AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. |