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RAC1/2/3 Polyclonal antibody

RAC1/2/3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16117-1-AP

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Synonyms

RAC3, Ras-related C3 botulinum toxin substrate 3, p21-Rac3, p21 Rac3, EC:3.6.5.2



经过测试的应用

Positive WB detected inSH-SY5Y cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16117-1-AP targets RAC1/2/3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RAC1/2/3 fusion protein Ag9045 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
Synonyms RAC3, Ras-related C3 botulinum toxin substrate 3, p21-Rac3, p21 Rac3, EC:3.6.5.2
Calculated Molecular Weight 192 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC015197
Gene Symbol RAC3
Gene ID (NCBI) 5881
RRIDAB_2176154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.

实验方案

Product Specific Protocols
WB protocol for RAC1/2/3 antibody 16117-1-APDownload protocol
IHC protocol for RAC1/2/3 antibody 16117-1-APDownload protocol
IP protocol for RAC1/2/3 antibody 16117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer

F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.

Authors - Su Dong
mouseWB

Front Cell Neurosci

A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.

Authors - Sira A Franchi
  • KO Validated
humanWB

Mol Biol Cell

Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.

Authors - Brian J Rosenberg
  • KD Validated
humanWB

BMC Cancer

Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.

Authors - Gest Caroline C
mouseWB

Glycoconj J

Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide.

Authors - Erika Di Biase
humanWB

Asian Pac J Cancer Prev

Silencing of rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.

Authors - Tie-Qin Liu
  • KD Validated