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验证数据展示

  • WB analysis using 30042-1-AP

    WB analysis using 30042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30042-1-AP (RACGAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30042-1-AP

    IHC staining of mouse testis using 30042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30042-1-AP (RACGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 30042-1-AP

    IF Staining of MCF-7 using 30042-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RACGAP1 antibody (30042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30042-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30042-1-AP targets RACGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 RACGAP1 fusion protein Ag32468 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rac GTPase activating protein 1
别名 HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1
计算分子量 632 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC032754
基因名称 RACGAP1
Gene ID (NCBI) 29127
RRIDAB_3086216
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0H5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RACGAP1 antibody 30042-1-APDownload protocol
IHC protocol for RACGAP1 antibody 30042-1-APDownload protocol
IF protocol for RACGAP1 antibody 30042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols