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- KD/KO Validated
RACK1; GNB2L1 Polyclonal antibody
RACK1; GNB2L1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (3)
Applications
WB, IHC, ELISA and More (4)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, L02 cells, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RACK1 Ready-To-Use IHC Kit. RACK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27592-1-AP targets RACK1; GNB2L1 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, RIP, CoIP |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, monkey, pig |
| Immunogen | RACK1; GNB2L1 fusion protein Ag26708 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 |
| Synonyms | Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC019093 |
| Gene Symbol | RACK1 |
| Gene ID (NCBI) | 10399 |
| RRID | AB_2880917 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63244 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RACK1; GNB2L1 antibody 27592-1-AP | Download protocol |
| IHC protocol for RACK1; GNB2L1 antibody 27592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages | ||
J Virol The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association | ||
Cancers (Basel) RACK1 Promotes Meningioma Progression by Activation of NF-κB Pathway via Preventing CSNK2B from Ubiquitination Degradation | ||
Virology RACK1 promotes porcine reproductive and respiratory syndrome virus infection in Marc-145 cells through ERK1/2 activation
| ||
Vet Microbiol Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis. |
