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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66940-1-Ig (same clone as 66940-1-PBS)

    WB analysis using 66940-1-Ig (same clone as 66940-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RACK1 antibody included
  • IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RACK1 antibody included
  • IF Staining of HeLa using 66940-1-Ig (same clone as 66940-1-PBS)

    IF Staining of HeLa using 66940-1-Ig (same clone as 66940-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RACK1; GNB2L1 antibody (66940-1-Ig, Clone: 1E11A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

RACK1; GNB2L1 Monoclonal antibody, PBS Only

RACK1; GNB2L1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66940-1-PBS

产品说明书

CloneNo. 1E11A7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RACK1 Ready-To-Use IHC Kit. RACK1 primary antibody included.

产品信息

66940-1-PBS targets RACK1; GNB2L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 RACK1; GNB2L1 fusion protein Ag26708 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
别名 Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1
计算分子量 36 kDa
观测分子量 32 kDa
GenBank蛋白编号BC019093
基因名称 RACK1
Gene ID (NCBI) 10399
RRIDAB_2882264
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63244
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).