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RACK1; GNB2L1 Monoclonal antibody, PBS Only

RACK1; GNB2L1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E11A7

Cat No : 66940-1-PBS

Print datasheet

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

验证数据展示

  • WB analysis using 66940-1-Ig (same clone as 66940-1-PBS)

    WB analysis using 66940-1-Ig (same clone as 66940-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66940-1-Ig (same clone as 66940-1-PBS)

    IF Staining of HeLa using 66940-1-Ig (same clone as 66940-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RACK1; GNB2L1 antibody (66940-1-Ig, Clone: 1E11A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

产品信息

66940-1-PBS targets RACK1; GNB2L1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen RACK1; GNB2L1 fusion protein Ag26708 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Synonyms Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC019093
Gene Symbol GNB2L1
Gene ID (NCBI) 10399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63244
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).