验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue, Raji cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11255-1-AP targets RAD51AP1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF, ChIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RAD51AP1 fusion protein Ag1781 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAD51 associated protein 1 |
别名 | RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51 |
计算分子量 | 38 kDa |
观测分子量 | 38 kDa |
GenBank蛋白编号 | BC016330 |
基因名称 | RAD51AP1 |
Gene ID (NCBI) | 10635 |
RRID | AB_2300786 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96B01 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].
实验方案
Product Specific Protocols | |
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WB protocol for RAD51AP1 antibody 11255-1-AP | Download protocol |
IF protocol for RAD51AP1 antibody 11255-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature RNA transcripts stimulate homologous recombination by forming DR-loops.
| ||
Mol Cell TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch | ||
Oncogene BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair. | ||
Front Genet Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis | ||
J Cell Biochem The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes. |