• Featured Product
  • KD/KO验证

RAD51AP1 Polyclonal antibody

RAD51AP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11255-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (2)

RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, mouse liver tissue, rat liver tissue, Raji cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11255-1-AP targets RAD51AP1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RAD51AP1 fusion protein Ag1781 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD51 associated protein 1
别名 RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC016330
基因名称 RAD51AP1
Gene ID (NCBI) 10635
RRIDAB_2300786
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

实验方案

Product Specific Protocols
WB protocol for RAD51AP1 antibody 11255-1-APDownload protocol
IF protocol for RAD51AP1 antibody 11255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nature

RNA transcripts stimulate homologous recombination by forming DR-loops.

Authors - Jian Ouyang
  • KD Validated
humanWB

Mol Cell

TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch

Authors - Tribhuwan Yadav
humanWB

Oncogene

BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.

Authors - Mengdong Ni
human,mouseIF

J Biol Chem

Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaks

Authors - Linlin Chen
humanWB

Front Genet

Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis

Authors - Renwang Liu
mouseWB

J Cell Biochem

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Authors - Shuhei Ishikura
Loading...