验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, MDA-MB-231 cells, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31678-1-AP targets RAD51C in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | RAD51C fusion protein Ag35070 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAD51 homolog C (S. cerevisiae) |
| 别名 | R51H3, RAD51 homolog C, RAD51 like protein 2, RAD51C, RAD51L2 |
| 计算分子量 | 376 aa, 42 kDa |
| 观测分子量 | 40-42 kDa |
| GenBank蛋白编号 | BC107753 |
| 基因名称 | RAD51C |
| Gene ID (NCBI) | 5889 |
| RRID | AB_3670072 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43502 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD51C antibody 31678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |