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  • Featured Product
  • KD/KO Validated

RAD52 Polyclonal antibody

RAD52 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 28045-1-AP

Print datasheet

Synonyms

RAD52, RAD52 homolog (S. cerevisiae)

验证数据展示

  • WB analysis using 28045-1-AP

    WB analysis using 28045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28045-1-AP (RAD52 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 28045-1-AP

    WB analysis of HepG2 using 28045-1-AP

    WB result of RAD52 antibody (28045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD52 transfected HepG2 cells.

  • IP experiment of HEK-293 using 28045-1-AP

    IP experiment of HEK-293 using 28045-1-AP

    IP result of anti-RAD52 (IP:28045-1-AP, 4ug; Detection:28045-1-AP 1:1000) with HEK-293 cells lysate 2960 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HL-60 cells, HeLa cells
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28045-1-AP targets RAD52 in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications IP, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, pig
Immunogen RAD52 fusion protein Ag27619 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD52 homolog (S. cerevisiae)
Synonyms RAD52, RAD52 homolog (S. cerevisiae)
Calculated Molecular Weight 46 aa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberNM_001297419
Gene Symbol RAD52
Gene ID (NCBI) 5893
RRIDAB_2881046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43351
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAD52 antibody 28045-1-APDownload protocol
IP protocol for RAD52 antibody 28045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Mol Cell

EXO1 protects BRCA1-deficient cells against toxic DNA lesions

Authors - Bert van de Kooij
View Article
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
View Article
humanWB

J Cell Mol Med

RDM1 promotes critical processes in breast cancer tumorigenesis.

Authors - Yajun Chen
View Article
pigWB

Int J Biochem Cell Biol

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Authors - Guoling Li
View Article
humanWB

Cell Death Dis

SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.

Authors - Yuchao Diao
  • KD Validated
View Article
humanWB

Nat Commun

Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity

Authors - Ronan Broderick
  • KD Validated
View Article