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RAD52 Polyclonal antibody

RAD52 Polyclonal Antibody for WB, IP, ELISA
Cat No. 28045-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, ELISA

DNA repair protein RAD52 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HL-60 cells, HepG2 cells
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28045-1-AP targets RAD52 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, pig
免疫原 RAD52 fusion protein Ag27619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD52 homolog (S. cerevisiae)
别名 DNA repair protein RAD52 homolog
计算分子量 46 aa
观测分子量 46-50 kDa
GenBank蛋白编号NM_001297419
基因名称 RAD52
Gene ID (NCBI) 5893
RRIDAB_2881046
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43351
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAD52 antibody 28045-1-APDownload protocol
IP protocol for RAD52 antibody 28045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Mol Cell

EXO1 protects BRCA1-deficient cells against toxic DNA lesions

Authors - Bert van de Kooij
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
humanWB

J Cell Mol Med

RDM1 promotes critical processes in breast cancer tumorigenesis.

Authors - Yajun Chen
pigWB

Int J Biochem Cell Biol

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Authors - Guoling Li
humanWB

Cell Death Dis

SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.

Authors - Yuchao Diao
  • KD Validated
humanWB

Nat Commun

Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity

Authors - Ronan Broderick
  • KD Validated
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