验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse thymus tissue |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
11825-1-AP targets RAG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | RAG2 fusion protein Ag2393 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | recombination activating gene 2 |
| Synonyms | V(D)J recombination-activating protein 2, RAG-2, RAG 2 |
| Calculated Molecular Weight | 527 aa, 59 kDa |
| Observed Molecular Weight | 57-62 kDa |
| GenBank Accession Number | BC022397 |
| Gene Symbol | RAG2 |
| Gene ID (NCBI) | 5897 |
| RRID | AB_2238062 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55895 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAG2 antibody 11825-1-AP | Download protocol |
| IHC protocol for RAG2 antibody 11825-1-AP | Download protocol |
| IF protocol for RAG2 antibody 11825-1-AP | Download protocol |
| IP protocol for RAG2 antibody 11825-1-AP | Download protocol |
| FC protocol for RAG2 antibody 11825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunol Allelic Exclusion of IgH through Inhibition of E2A in a VDJ Recombination Complex. | ||
Hum Cell Assessment of non-myelotoxic agents as a preparatory regimen for hematopoietic stem cell gene therapy |
