RAG2 Polyclonal antibody

RAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11825-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

V(D)J recombination-activating protein 2, RAG-2, RAG 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, mouse thymus tissue
Positive IP detected inA375 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11825-1-AP targets RAG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 RAG2 fusion protein Ag2393 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 recombination activating gene 2
别名 V(D)J recombination-activating protein 2, RAG-2, RAG 2
计算分子量 527 aa, 59 kDa
观测分子量 57-62 kDa
GenBank蛋白编号BC022397
基因名称 RAG2
Gene ID (NCBI) 5897
RRIDAB_2238062
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55895
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

实验方案

Product Specific Protocols
WB protocol for RAG2 antibody 11825-1-APDownload protocol
IHC protocol for RAG2 antibody 11825-1-APDownload protocol
IF protocol for RAG2 antibody 11825-1-APDownload protocol
IP protocol for RAG2 antibody 11825-1-APDownload protocol
FC protocol for RAG2 antibody 11825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

The 3D enhancer network of the developing T cell genome is shaped by SATB1

Authors - Tomas Zelenka
mouseWB

J Immunol

Allelic Exclusion of IgH through Inhibition of E2A in a VDJ Recombination Complex.

Authors - Jannek Hauser
mouseIF

Hum Cell

Assessment of non-myelotoxic agents as a preparatory regimen for hematopoietic stem cell gene therapy

Authors - Mehmet Emin Şeker
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