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  • Featured Product
  • KD/KO Validated

MOK Polyclonal antibody

MOK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23926-1-AP

Print datasheet

Synonyms

MOK, MOK protein kinase, RAGE, RAGE 1, RAGE1, renal tumor antigen, Renal tumor antigen 1

验证数据展示

  • WB analysis of HeLa using 23926-1-AP

    WB analysis of HeLa using 23926-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 23926-1-AP

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 23926-1-AP

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 23926-1-AP

    IF Staining of HeLa using 23926-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23926-1-AP is MOK Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MOK fusion protein Ag20849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name renal tumor antigen
Synonyms MOK, MOK protein kinase, RAGE, RAGE 1, RAGE1, renal tumor antigen, Renal tumor antigen 1
Calculated Molecular Weight 231 aa, 27 kDa
Observed Molecular Weight 42 kDa, 85 kDa
GenBank Accession NumberBC053536
Gene Symbol RAGE
Gene ID (NCBI) 5891
RRIDAB_2879364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MOK antibody 23926-1-APDownload protocol
IHC protocol for MOK antibody 23926-1-APDownload protocol
IF protocol for MOK antibody 23926-1-APDownload protocol
FC protocol for MOK antibody 23926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities

Authors - Jing Hang
  • KD Validated
View Article
WB

J Biol Chem

Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.

Authors - Ran Li
View Article