验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17507-1-AP targets RAI14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | RAI14 fusion protein Ag11708 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoic acid induced 14 |
| 别名 | retinoic acid induced 14, RAI13, NORPEG, KIAA1334, Ankyrin repeat and coiled-coil structure-containing protein |
| 计算分子量 | 980 aa, 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC052988 |
| 基因名称 | RAI14 |
| Gene ID (NCBI) | 26064 |
| RRID | AB_2175992 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P0K7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IHC protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IF protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IP protocol for RAI14 antibody 17507-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein | ||
Elife Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors. | ||
Cells Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model. | ||
Cell Transplant RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer. | ||
PLoS One Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.
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