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RAI2 Polyclonal antibody

RAI2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28217-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28217-1-AP

    WB analysis using 28217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28217-1-AP (RAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, IMR-32 cells, SK-N-SH cells, TT cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28217-1-AP targets RAI2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RAI2 fusion protein Ag28253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoic acid induced 2
Synonyms
Calculated Molecular Weight 57 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC027937
Gene Symbol RAI2
Gene ID (NCBI) 10742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retinoic acid-induced 2 (RAI2) was initially described as a retinoic acid-inducible gene, which has been assumed to be involved in development. RAI2 was initially related to cell differentiation and induced by retinoic acid and has been identified as an emerging tumor suppressor in breast cancer and colorectal cancer. RAI2 is frequently methylated in human CRC, and the expression of RAI2 is regulated by promoter region methylation (PMID: 29796120).

实验方案

Product Specific Protocols
WB protocol for RAI2 antibody 28217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols