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验证数据展示

  • WB analysis of K-562 using 13176-1-AP

    WB analysis of K-562 using 13176-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13176-1-AP

    IP experiment of K-562 using 13176-1-AP

    IP result of anti-RALBP1 (IP:13176-1-AP, 4ug; Detection:13176-1-AP 1:800) with K-562 cells lysate 3600ug.

  • IHC staining of human stomach using 13176-1-AP

    IHC staining of human stomach using 13176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13176-1-AP (RALBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALBP1 antibody included

RALBP1 Polyclonal antibody

RALBP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13176-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

EC:7.6.2.2, EC:7.6.2.3, DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, HepG2 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13176-1-AP targets RALBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 RALBP1 fusion protein Ag3843 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ralA binding protein 1
别名 EC:7.6.2.2, EC:7.6.2.3, DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein
计算分子量 655 aa, 76 kDa
观测分子量 95 kDa
GenBank蛋白编号BC013126
基因名称 RALBP1
Gene ID (NCBI) 10928
RRIDAB_2176171
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALBP1 antibody 13176-1-APDownload protocol
IHC protocol for RALBP1 antibody 13176-1-APDownload protocol
IP protocol for RALBP1 antibody 13176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

PMID: 28092822

Authors - Jian Yang
View Article