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RALBP1 Polyclonal antibody

RALBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13176-1-AP

Print datasheet

Synonyms

EC:7.6.2.2, EC:7.6.2.3, DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein

验证数据展示

  • WB analysis of K-562 using 13176-1-AP

    WB analysis of K-562 using 13176-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13176-1-AP

    IP experiment of K-562 using 13176-1-AP

    IP result of anti-RALBP1 (IP:13176-1-AP, 4ug; Detection:13176-1-AP 1:800) with K-562 cells lysate 3600ug.

  • IHC staining of human stomach using 13176-1-AP

    IHC staining of human stomach using 13176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13176-1-AP (RALBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALBP1 antibody included

经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, HepG2 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13176-1-AP targets RALBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen RALBP1 fusion protein Ag3843 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ralA binding protein 1
Synonyms EC:7.6.2.2, EC:7.6.2.3, DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein
Calculated Molecular Weight 655 aa, 76 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC013126
Gene Symbol RALBP1
Gene ID (NCBI) 10928
RRIDAB_2176171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15311
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALBP1 antibody 13176-1-APDownload protocol
IHC protocol for RALBP1 antibody 13176-1-APDownload protocol
IP protocol for RALBP1 antibody 13176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

PMID: 28092822

Authors - Jian Yang
View Article