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RALYL Monoclonal antibody

RALYL Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3F4

Cat no : 67534-1-Ig

Print datasheet

Synonyms

hRALYL, HNRPCL3, hnRNP core protein C-like 3, hnRNP core protein C like 3, Heterogeneous nuclear ribonucleoprotein C-like 3

验证数据展示

  • WB analysis using 67534-1-Ig

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67534-1-Ig

    IF Staining of HepG2 using 67534-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67534-1-Ig (RALYL antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67534-1-Ig

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67534-1-Ig

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig brain tissue, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, LNCaP cells, IMR-32 cells, Daudi cells, NK-92 cells, HepG2 cells, Raji cells, rabbit brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67534-1-Ig targets RALYL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen RALYL fusion protein Ag11676 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RALY RNA binding protein-like
Synonyms hRALYL, HNRPCL3, hnRNP core protein C-like 3, hnRNP core protein C like 3, Heterogeneous nuclear ribonucleoprotein C-like 3
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC031090
Gene Symbol RALYL
Gene ID (NCBI) 138046
RRIDAB_2882753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86SE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALYL antibody 67534-1-IgDownload protocol
IF protocol for RALYL antibody 67534-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols