验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HeLa cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue, HEK-293 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue, human colon tissue, human placenta tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, macrobrachium rosenbergii |
| Immunogen | RAN fusion protein Ag0748 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RAN, member RAS oncogene family |
| Synonyms | ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, GTP binding nuclear protein Ran |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC004272 |
| Gene Symbol | RAN |
| Gene ID (NCBI) | 5901 |
| RRID | AB_2176484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62826 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAN antibody 10469-1-AP | Download protocol |
| IHC protocol for RAN antibody 10469-1-AP | Download protocol |
| IF protocol for RAN antibody 10469-1-AP | Download protocol |
| IP protocol for RAN antibody 10469-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.
| ||
Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. | ||
Cell Commun Signal Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. | ||
Oncotarget Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS. | ||
Int J Biol Sci Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.
| ||
J Cell Sci A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex. |
